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人白介素1可溶性受體Ⅱ酶免試劑盒,(IL-1sRⅡ)ELISA檢測試劑盒,英文名:IL-1sRⅡ ELISA Kit,英文縮寫:IL-1sRⅡ ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人白介素1可溶性受體ⅡELISA檢測試劑盒,IL-1sRⅡ 檢測試劑盒,IL-1sRⅡ 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人白介素1可溶性受體Ⅱ酶免試劑盒,(IL-1sRⅡ)ELISA檢測試劑盒 操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
兔鉤端螺旋體IgG(Lep IgG)ELISA試劑盒Rabbit Leptospira IgG,Lep IgG ELISA Kit Y0310196T/48T
兔凝血酶原片段F1+2(F1+2)ELISA試劑盒Rabbit prothrombin fragment 1+2,F1+2 ELISA Kit Y0310296T/48T
兔子腎上腺髓質(zhì)素(ADM)ELISA試劑盒Rabbit soluble endothelial protein C receptor,sEPCR ELISA KitY0310396T/48T
兔子可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒Rabbit soluble endothelial protein C receptor,sEPCR ELISA KitY0310496T/48T
兔脂蛋白α(Lp-α)ELISA試劑盒 Rabbit lipoprotein α,Lp-α ELSIA Kit Y0310596T/48T
兔載脂蛋白E(Apo-E)ELISA試劑盒 Rabbit Apolipoprotein E,Apo-E ELISA Kit Y0310696T/48T
兔載脂蛋白B100(apo-B100)ELISA試劑盒 Rabbit apoprotein B100,apo-B100 ELISA Kit Y0310796T/48T
兔轉(zhuǎn)化生長因子β2(TGFβ2)ELISA試劑盒Rabbit transforming growth factorβ2,TGFβ2 ELISA KitY0310896T/48T
兔一氧化氮合成酶(NOS)ELISA試劑盒 Rabbit nitric oxide synthase,NOS ELISA Kit Y0310996T/48T
兔內(nèi)皮型一氧化氮合成酶(eNOS)ELISA試劑盒 Rabbit Endothelial nitric oxide synthase,eNOS ELISA Kit Y0311096T/48T
兔尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)ELISA試劑盒 Rabbit Urokinase-type Plasminogen Activator Receptor,PLAUR/uPAR ELISA Kit Y0311196T/48T
兔尿激酶型纖溶酶原激活物(uPA)ELISA試劑盒Rabbit urokinase plasminogen activator,uPA ELISA Kit Y0311296T/48T
兔超敏C反應(yīng)蛋白(hs-CRP)ELISA試劑盒Rabbit high sensitivity C-Reactive Protein,hs-CRP ELISA Kit Y0311396T/48T
兔基質(zhì)金屬蛋白酶1(MMP-1)ELISA試劑盒 Rabbit matrix metalloproteinase 1,MMP-1 ELISA Kit Y0311496T/48T
兔8-異構(gòu)前列腺素(8-epi-PGF2α)ELISA試劑盒Rabbit 8-epi-prostaglandin F2alpha,8-epi-PGF2α ELISA KitY0311596T/48T
兔子血小板因子4(PF-4/CXCL4)ELISA試劑盒Rabbit Plaet Factor 4,PF-4 ELISA KitY0311696T/48T
兔子β血小板球蛋白/β血栓環(huán)蛋白(β-TG)ELISA試劑盒Rabbit β-Thromboglobulin,β-TG ELISA KitY0311796T/48T
兔子中性粒細(xì)胞彈性蛋白酶(NE)ELISA試劑盒Rabbit neutrophil elastase,NE ELISA KitY0311896T/48T
兔子補體片斷3a(C3a)ELISA試劑盒Rabbit Complement fragment 3a,C3a ELISA KitY0311996T/48T
兔子基質(zhì)金屬蛋白酶3(MMP-3)ELISA試劑盒Rabbit matrix metalloproteinase 3,MMP-3 ELISA Kit Y0312096T/48T
兔子可溶性CD40配體(sCD40L)ELISA試劑盒differentiation 40 ligand,sCD40L ELISA KitY0312196T/48T
兔主要組織相容性復(fù)合體Ⅰ類(MHCⅠ/RLAⅠ)ELISA試劑盒 Rabbit major histocompatibility complexⅠ,MHCⅠ/RLAⅠ ELISA KitY0312296T/48T
兔子高遷移率族蛋白B1(HMGB-1)ELISA試劑盒 Rabbit High mobility group protein B1,HMGB-1 ELISA Kit Y0312396T/48T
兔子B細(xì)胞淋巴瘤因子2(Bcl-2)ELISA試劑盒Rabbit B-cell leukemia/lymphoma 2,Bcl-2 ELISA Kit Y0312496T/48T
兔色素上皮衍生因子(PEDF)ELISA試劑盒Rabbit pigment epithelium-derived factor,PEDF ELISA KitY0312596T/48T
兔抗糖蛋白抗體(GP)ELISA試劑盒Rabbit anti-glycoprotein antibody,GP ELISA KitY0312696T/48T
兔抗單核細(xì)胞抗體(AMA)ELISA試劑盒Rabbit anti-Monocyte antibody,AMA ELISA KitY0312796T/48T
兔氧化低密度脂蛋白抗體(OLAb)ELISA試劑盒Rabbit oxidized lowdensity lipoprotein antibody,OLAb ELISA KitY0312896T/48T
兔抗平滑肌抗體(ASMA) ELISA試劑盒Rabbit Anti-Smooth Muscle Antibody,ASMA ELISA KitY0312996T/48T
兔子白介素9(IL-9)ELISA試劑盒 Rabbit Interleukin 9,IL-9 ELISA Kit Y0313096T/48T
兔載脂蛋白A1(apo-A1)ELISA試劑盒 Rabbit apoprotein A1,apo-A1 ELISA Kit Y0313196T/48T
兔纖溶酶抗纖溶酶復(fù)合物(PAP)ELISA試劑盒 Rabbit plasmin-antiplasmin complex,PAP ELISA KitY0313296T/48T
兔纖溶抑制因子/凝血酶激活的纖溶抑制物(TAFI)ELISA試劑盒Rabbit Thrombin activatable fibrinolysis inhibitor,TAFI ELISA KitY0313396T/48T
兔子白介素2(IL-2)ELISA試劑盒Rabbit Interleukin 2,IL-2 ELISA KitY0313496T/48T
兔血漿α顆粒膜蛋白(GMP-140)ELISA試劑盒Rabbit alpha-granular membrane protein,GMP-140 ELISA KitY0313596T/48T
兔一氧化氮(NO)ELISA試劑盒Rabbit nitric oxide,NO ELISA Kit Y0313696T/48T
兔補體蛋白3(C3)ELISA試劑盒Rabbit Complement 3,C3 ELISA KitY0313796T/48T
兔p物質(zhì)(SP)ELISA試劑盒Rabbit Substance P,SP ELISA KitY0313896T/48T
兔子層連蛋白/板層素(LN)ELISA試劑盒Rabbit Laminin,LN ELISA KitY0313996T/48T
兔環(huán)磷酸腺苷(cAMP)ELISA試劑盒Rabbit cyclic adenosine monophosphate,cAMP ELISA KitY0314096T/48T
兔血管性血友病因子/瑞斯托霉素輔因子(VWF)ELISA試劑盒Rabbit von Willebrand Factor,VWF ELISA KitY0314196T/48T
兔血管內(nèi)皮細(xì)胞生長因子(VEGF)ELISA試劑盒 Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA KitY0314296T/48T
兔骨橋素(OPN)ELISA試劑盒Rabbit Osteopontin,OPN ELISA Kit Y0314396T/48T
兔子脂蛋白磷脂酶A2(Lp-PL-A2)ELISA試劑盒Rabbit lipoprotein-associated phospholipase A2,Lp-PL-A2 ELISA KitY0314496T/48T
兔血清總補體(CH50)ELISA試劑盒Rabbit 50% complement hemolysis,CH50 ELISA KitY0314596T/48T
兔乙酰膽堿受體抗體(AChRab)ELISA試劑盒Rabbit acetylcholine receptor antibody,AChRab ELISA Kit Y0314696T/48T
兔誘導(dǎo)型一氧化氮合成酶(iNOS)ELISA試劑盒Rabbit inducible nitric oxide synthase,iNOS ELISA Kit Y0314796T/48T
兔熱休克蛋白90(HSP-90)ELISA試劑盒Rabbit Heat Shock Protein 90,HSP-90 ELISA KitY0314896T/48T
兔熱休克蛋白70(HSP-70)ELISA試劑盒
Rabbit Heat Shock Protein 70,HSP-70 ELISA KitY0314996T/48T
兔熱休克蛋白40(HSP-40)ELISA試劑盒Rabbit Heat Shock Protein 40,HSP-40 ELISA KitY0315096T/48T
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。