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小鼠白介素13酶免試劑盒,(IL-13)ELISA檢測試劑盒,英文名:IL-13 ELISA Kit,英文縮寫:IL-13 ,常見試劑盒規格:96T/48T,產品別名:小鼠白介素13ELISA檢測試劑盒,IL-13 檢測試劑盒,IL-13 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠白介素13酶免試劑盒,(IL-13)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
內皮素轉化酶1(ECE1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endothelin Converting Enzyme 1 (ECE1)48T/96TY10485Homo sapiens (Human)
內皮素轉化酶1(ECE1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endothelin Converting Enzyme 1 (ECE1)48T/96TY10486Rattus norvegicus (Rat)
內皮素轉化酶1(ECE1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endothelin Converting Enzyme 1 (ECE1)48T/96TY10487Mus musculus (Mouse)
氨基端前心鈉肽(NT-ProANP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Terminal Pro Atrial Natriuretic Peptide (NT-ProANP)48T/96TY10488Homo sapiens (Human)
氨基端前心鈉肽(NT-ProANP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Terminal Pro Atrial Natriuretic Peptide (NT-ProANP)48T/96TY10489Rattus norvegicus (Rat)
氨基端前心鈉肽(NT-ProANP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Terminal Pro Atrial Natriuretic Peptide (NT-ProANP)48T/96TY10490Mus musculus (Mouse)
氨基端前腦鈉素(NT-ProBNP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP)48T/96TY10491Homo sapiens (Human)
氨基端前腦鈉素(NT-ProBNP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP)48T/96TY10492Mus musculus (Mouse)
D-二聚體(D2D)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for D-Dimer (D2D)48T/96TY10493Homo sapiens (Human)
蛋白激酶D1(PRKD1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Kinase D1 (PKD1)48T/96TY10494Homo sapiens (Human)
鐵蛋白(FE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ferritin (FE)48T/96TY10495Homo sapiens (Human)
淋巴細胞功能關聯抗原2(LFA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lymphocyte Function Associated Antigen 2 (LFA2)48T/96TY10496Homo sapiens (Human)
組織因子(TF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tissue Factor (TF)48T/96TY10497Sus scrofa; Porcine (Pig)
組織型纖溶酶原激活因子(tPA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plasminogen Activator, Tissue (tPA)48T/96TY10498Sus scrofa; Porcine (Pig)
血栓調節蛋白(TM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thrombomodulin (TM)48T/96TY10499Canis familiaris; Canine (Dog)
血栓調節蛋白(TM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thrombomodulin (TM)48T/96TY10500Sus scrofa; Porcine (Pig)
纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1)48T/96TY10501Sus scrofa; Porcine (Pig)
細胞間粘附分子3(ICAM3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 3 (ICAM3)48T/96TY10502Sus scrofa; Porcine (Pig)
細胞間粘附分子2(ICAM2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2)48T/96TY10503Sus scrofa; Porcine (Pig)
白介素2(IL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 2 (IL2)48T/96TY10504Chicken (Gallus)
白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 4 (IL4)48T/96TY10505Chicken (Gallus)
干擾素γ(IFNγ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY10506Cavia (Guinea pig )
內皮素1(EDN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10507Cavia (Guinea pig )
生長激素(GH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Hormone (GH)48T/96TY10508Cavia (Guinea pig )
瘦素(LEP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Leptin (LEP)48T/96TY10509Bos taurus; Bovine (Cattle)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY10510Cavia (Guinea pig )
轉化生長因子β1(TGFβ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)48T/96TY10511Cavia (Guinea pig )
Ⅲ型膠原(COL3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type III (COL3)48T/96TY10512Sus scrofa; Porcine (Pig)
白介素10(IL10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 10 (IL10)48T/96TY10513Sus scrofa; Porcine (Pig)
白介素18(IL18)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 18 (IL18)48T/96TY10514Sus scrofa; Porcine (Pig)
白介素1β(IL1β)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY10515Sus scrofa; Porcine (Pig)
白介素2(IL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 2 (IL2)48T/96TY10516Sus scrofa; Porcine (Pig)
白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 4 (IL4)48T/96TY10517Sus scrofa; Porcine (Pig)
白介素6(IL6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 6 (IL6)48T/96TY10518Sus scrofa; Porcine (Pig)
白介素8(IL8)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 8 (IL8)48T/96TY10519Sus scrofa; Porcine (Pig)
表皮生長因子(EGF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Epidermal Growth Factor (EGF)48T/96TY10520Sus scrofa; Porcine (Pig)
促生長激素釋放激素(GHRH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Hormone Releasing Hormone (GHRH)48T/96TY10521Sus scrofa; Porcine (Pig)
干擾素γ(IFNγ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY10522Sus scrofa; Porcine (Pig)
基質金屬蛋白酶9(MMP9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 9 (MMP9)48T/96TY10523Sus scrofa; Porcine (Pig)
內皮素1(EDN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10524Sus scrofa; Porcine (Pig)
腦鈉素(BNP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Brain Natriuretic Peptide (BNP)48T/96TY10525Sus scrofa; Porcine (Pig)
生長激素(GH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Hormone (GH)48T/96TY10526Sus scrofa; Porcine (Pig)
生長抑素(SST)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Somatostatin (SST)48T/96TY10527Sus scrofa; Porcine (Pig)
瘦素(LEP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Leptin (LEP)48T/96TY10528Sus scrofa; Porcine (Pig)
β-內啡肽(βEP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Beta-Endorphin (bEP)48T/96TY10529Homo sapiens (Human)
超氧化物歧化酶1(SOD1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Superoxide Dismutase 1, Soluble (SOD1)48T/96TY10530Cavia (Guinea pig )
β-內啡肽(βEP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Beta-Endorphin (bEP)48T/96TY10531Mus musculus (Mouse)
胰島素(INS)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Insulin (INS)48T/96TY10532Sus scrofa; Porcine (Pig)
胰島素樣生長因子1(IGF1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY10533Sus scrofa; Porcine (Pig)
胰島素樣生長因子2(IGF2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 2 (IGF2)48T/96TY10534Sus scrofa; Porcine (Pig)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。