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您當前的位置:首頁 > 產品展示 > ELISA檢測試劑盒 > 小鼠ELISA檢測試劑盒 > 小鼠白介素18酶免試劑盒,(IL-18)ELISA檢測試劑盒
小鼠白介素18酶免試劑盒,(IL-18)ELISA檢測試劑盒
產品時間:2016-09-29
小鼠白介素18酶免試劑盒,(IL-18)ELISA檢測試劑盒,行業*,國內外多家ELISA試劑盒代理供應,價格相對平價,公司提供免費代測及ELISA試劑盒包郵服務。

本產品僅用于科研,不用于臨床

小鼠白介素18酶免試劑盒,(IL-18)ELISA檢測試劑盒,英文名:IL-18 ELISA Kit,英文縮寫:IL-18 ,常見試劑盒規格:96T/48T,產品別名:小鼠白介素18ELISA檢測試劑盒,IL-18 檢測試劑盒,IL-18 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠白介素18酶免試劑盒,(IL-18)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。   

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S100鈣結合蛋白B(S100B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein B (S100B)48T/96TY10569Oryctolagus cuniculus (Rabbit)
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白血病抑制因子受體(LIFR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Leukemia Inhibitory Factor Receptor (LIFR)48T/96TY10575Oryctolagus cuniculus (Rabbit)
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干擾素γ(IFNγ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY10583Oryctolagus cuniculus (Rabbit)
肝細胞生長因子(HGF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hepatocyte Growth Factor (HGF)48T/96TY10584Oryctolagus cuniculus (Rabbit) 

各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。

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