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小鼠白介素1α酶免試劑盒,(IL-1α)ELISA檢測試劑盒,英文名:IL-1α ELISA Kit,英文縮寫:IL-1α ,常見試劑盒規格:96T/48T,產品別名:小鼠白介素1αELISA檢測試劑盒,IL-1α 檢測試劑盒,IL-1α 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠白介素1α酶免試劑盒,(IL-1α)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
干擾素γ誘導蛋白16(IFI16)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Gamma Inducible Protein 16 (IFI16)48T/96TY11035Homo sapiens (Human)
Toll樣受體4(TLR4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Toll Like Receptor 4 (TLR4)48T/96TY11036Mus musculus (Mouse)
Toll樣受體2(TLR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Toll Like Receptor 2 (TLR2)48T/96TY11037Mus musculus (Mouse)
激活素受體樣激酶1(ALK1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Activin Receptor Like Kinase 1 (ALK1)48T/96TY11038Homo sapiens (Human)
心肌鈣黏蛋白(CDHH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cadherin, Heart (CDHH)48T/96TY11039Homo sapiens (Human)
纖維膠凝蛋白3(FCN3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ficolin 3 (FCN3)48T/96TY11040Homo sapiens (Human)
檸檬酸合酶(CS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Citrate Synthase (CS)48T/96TY11041Homo sapiens (Human)
血小板膜糖蛋白Ⅵ(GP6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycoprotein VI, Plaet (GP6)48T/96TY11042Homo sapiens (Human)
白介素7受體(IL7R)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 7 Receptor (IL7R)48T/96TY11043Rattus norvegicus (Rat)
白介素28受體(IL28R)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 28 Receptor (IL28R)48T/96TY11044Rattus norvegicus (Rat)
Ⅱ型膠原交聯羧基端肽(CTXⅡ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cross Linked C-opeptide Of Type II Collagen (CTXII)48T/96TY11045Rattus norvegicus (Rat)
軟骨寡聚基質蛋白(COMP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cartilage Oligomeric Matrix Protein (COMP)48T/96TY11046Rattus norvegicus (Rat)
纖維膠凝蛋白2(FCN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ficolin 2 (FCN2)48T/96TY11047Homo sapiens (Human)
聚集蛋白聚糖(AGC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aggrecan (AGC)48T/96TY11048Homo sapiens (Human)
p53上調凋亡調節因子(PUMA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for p53 Upregulated Modulator Of Apoptosis (PUMA)48T/96TY11049Homo sapiens (Human)
血小板親和蛋白1(PKP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plakophilin 1 (PKP1)48T/96TY11050Homo sapiens (Human)
激肽釋放酶7(KLK7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kallikrein 7 (KLK7)48T/96TY11051Homo sapiens (Human)
S抗原(SAG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for S Antigen (SAG)48T/96TY11052Homo sapiens (Human)
蛋白激酶Cζ(PKCζ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Kinase C Zeta (PKCz)48T/96TY11053Rattus norvegicus (Rat)
白介素5(IL5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 5 (IL5)48T/96TY11054Cavia (Guinea pig )
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4)48T/96TY11055Rattus norvegicus (Rat)
Smad同源物4(Smad4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 4 (Smad4)48T/96TY11056Homo sapiens (Human)
趨化因子C-C-基元受體4(CCR4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Receptor 4 (CCR4)48T/96TY11057Homo sapiens (Human)
血管生成素樣蛋白2(ANGPTL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 2 (ANGPTL2)48T/96TY11058Homo sapiens (Human)
干擾素γ誘導單核因子(MIγ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Monokine Induced By Interferon Gamma (MIg)48T/96TY11059Homo sapiens (Human)
蛋白激酶Cα(PKCα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Kinase C Alpha (PKCa)48T/96TY11060Rattus norvegicus (Rat)
防御素α5(DEFα5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Defensin Alpha 5, Paneth Cell Specific (DEFa5)48T/96TY11061Homo sapiens (Human)
溶菌酶(LZM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lysozyme (LZM)48T/96TY11062Chicken (Gallus)
煙堿型膽堿受體α4(CHRNα4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4)48T/96TY11063Homo sapiens (Human)
甲酰肽受體2(FPR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Formyl Peptide Receptor 2 (FPR2)48T/96TY11064Homo sapiens (Human)
布魯東氏酪氨酸激酶(Btk)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Bruton'S Tyrosine Kinase (Btk)48T/96TY11065Homo sapiens (Human)
磷脂酰絲氨酸受體(PSR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phosphatidylserine Receptor (PSR)48T/96TY11066Homo sapiens (Human)
免疫缺陷伴血小板減少綜合征蛋白(WASP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Wiskott Aldrich Syndrome Protein (WASP)48T/96TY11067Homo sapiens (Human)
成纖維細胞生長因子7(FGF7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 7 (FGF7)48T/96TY11068Rattus norvegicus (Rat)
環加氧酶2(PTGS2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)48T/96TY11069Rattus norvegicus (Rat)
轉錄激活因子5(ATF5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Activating Transcription Factor 5 (ATF5)48T/96TY11070Mus musculus (Mouse)
膽堿乙酰轉移酶(ChAT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Choline Acetyltransferase (ChAT)48T/96TY11071Rattus norvegicus (Rat)
前列腺素D2(PGD2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prostaglandin D2 (PGD2)48T/96TY11072General
丙酮酸脫氫酶β(PDHβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pyruvate Dehydrogenase Beta (PDHb)48T/96TY11073Homo sapiens (Human)
煙堿型膽堿受體α2(CHRNα2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 2 (CHRNa2)48T/96TY11074Homo sapiens (Human)
脫鐵轉鐵蛋白(apoTf)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apotransferrin (apoTf)48T/96TY11075Homo sapiens (Human)
延胡索酸酶(FUM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fumarase (FUM)48T/96TY11076Homo sapiens (Human)
甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)48T/96TY11077Homo sapiens (Human)
成纖維細胞生長因子23(FGF23)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 23 (FGF23)48T/96TY11078Rattus norvegicus (Rat)
谷丙轉氨酶(ALT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alanine Aminotransferase (ALT)48T/96TY11079Rattus norvegicus (Rat)
激肽釋放酶10(KLK10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kallikrein 10 (KLK10)48T/96TY11080Rattus norvegicus (Rat)
組織蛋白酶S(CTSS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cathepsin S (CTSS)48T/96TY11081Homo sapiens (Human)
白介素32(IL32)檢測試劑盒(酶聯免疫吸附試驗法)小鼠白介素1α酶免試劑盒,(IL-1α)ELISA檢測試劑盒ELISA Kit for Interleukin 32 (IL32)48T/96TY11082Homo sapiens (Human)
亮氨酸豐富α2-糖蛋白1(LRG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)48T/96TY11083Homo sapiens (Human)
肌腱蛋白X(TNX)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tenascin X (TNX)48T/96TY11084Homo sapiens (Human)
各種類型ELISA測定時,小鼠白介素1α酶免試劑盒,(IL-1α)ELISA檢測試劑盒一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。