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小鼠Ⅰ型膠原交聯羧基末端肽酶免試劑盒,(ⅠCTP)ELISA檢測試劑盒 ,英文名:ⅠCTP ELISA Kit ,英文縮寫:ⅠCTP ,常見試劑盒規格:96T/48T,產品別名:小鼠Ⅰ型膠原交聯羧基末端肽ELISA檢測試劑盒,ⅠCTP 檢測試劑盒, ⅠCTP 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠Ⅰ型膠原交聯羧基末端肽酶免試劑盒,(ⅠCTP)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
穿孔素1(PRF1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Perforin 1 (PRF1)48T/96TY12335Mus musculus (Mouse)
骨膜蛋白(POSTN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Periostin (POSTN)48T/96TY12336Homo sapiens (Human)
腦源性神經營養因子(BDNF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)48T/96TY12337Oryctolagus cuniculus (Rabbit)
雄烯酮(ADT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Androsterone (ADT)48T/96TY12338General
α2-巨球蛋白(α2M)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alpha-2-Macroglobulin (a2M)48T/96TY12339Mus musculus (Mouse)
泛素結合酶E2A(UBE2A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ubiquitin Conjugating Enzyme E2A (UBE2A)48T/96TY12340Mus musculus (Mouse)
Axis抑制蛋白2(AXIN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Axis Inhibition Protein 2 (AXIN2)48T/96TY12341Homo sapiens (Human)
端錨聚合酶1(TNKS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tankyrase 1 (TNKS1)48T/96TY12342Homo sapiens (Human)
端錨聚合酶2(TNKS2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tankyrase 2 (TNKS2)48T/96TY12343Homo sapiens (Human)
肌纖蛋白(MYOC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myocilin (MYOC)48T/96TY12344Homo sapiens (Human)
肉毒堿棕櫚?;D移酶1A(CPT1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carnitine Palmitoyltransferase 1A, Liver (CPT1A)48T/96TY12345Homo sapiens (Human)
叉頭框蛋白A2(FOXA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Forkhead Box Protein A2 (FOXA2)48T/96TY12346Mus musculus (Mouse)
回腸脂肪酸結合蛋白(FABP6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 6, Ileal (FABP6)48T/96TY12347Mus musculus (Mouse)
白細胞關聯免疫球蛋白樣受體2(LAIR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Leukocyte Associated Immunoglobulin Like Receptor 2 (LAIR2)48T/96TY12348Homo sapiens (Human)
核仁素(NCL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleolin (NCL)48T/96TY12349Mus musculus (Mouse)
染色框同源物3(CBX3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chromobox Homolog 3 (CBX3)48T/96TY12350Mus musculus (Mouse)
Cathelicidin抗菌肽(CAMP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP)48T/96TY12351Homo sapiens (Human)
核仁磷酸蛋白(NPM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleophosmin (NPM)48T/96TY12352Mus musculus (Mouse)
2',5'-寡腺苷酸合成酶1(OAS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 2',5'-Oligoadenylate Synthetase 1 (OAS1)48T/96TY12353Mus musculus (Mouse)
神經纖維網蛋白1(NRP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neuropilin 1 (NRP1)48T/96TY12354Mus musculus (Mouse)
戊糖素(PTD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pentosidine (PTD)48T/96TY12355General
血管抑素(ANG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Angiostatin (ANG)48T/96TY12356Mus musculus (Mouse)
密封蛋白(OCLN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Occludin (OCLN)48T/96TY12357Homo sapiens (Human)
封閉蛋白1(CLDN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Claudin 1 (CLDN1)48T/96TY12358Homo sapiens (Human)
周期素依賴性激酶抑制因子1A(CDKN1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A)48T/96TY12359Mus musculus (Mouse)
封閉蛋白4(CLDN4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Claudin 4 (CLDN4)48T/96TY12360Homo sapiens (Human)
成纖維細胞生長因子7(FGF7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 7 (FGF7)48T/96TY12361Mus musculus (Mouse)
血管活性腸肽受體1(VIPR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vasoactive Intestinal Peptide Receptor 1 (VIPR1)48T/96TY12362Homo sapiens (Human)
失調蛋白1(ATXN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ataxin 1 (ATXN1)48T/96TY12363Homo sapiens (Human)
Ⅵ型膠原α1(COL6α1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type VI Alpha 1 (COL6a1)48T/96TY12364Homo sapiens (Human)
中腦星形膠質細胞來源神經營養因子(MANF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)48T/96TY12365Homo sapiens (Human)
酸性葡萄糖苷酶β(GβA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glucosidase Beta, Acid (GbA)48T/96TY12366Homo sapiens (Human)
Syncollin蛋白(SYCN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Syncollin (SYCN)48T/96TY12367Homo sapiens (Human)
Thy1細胞表面抗原(Thy1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thy1 Cell Surface Antigen (Thy1)48T/96TY12368Mus musculus (Mouse)
α1-酸性糖蛋白(α1AGP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)48T/96TY12369Sus scrofa; Porcine (Pig)
P-選擇素糖蛋白配體1(PSGL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for P-Selectin Glycoprotein Ligand 1 (PSGL1)48T/96TY12370Mus musculus (Mouse)
腫瘤壞死因子配體超家族成員7(TNFSF7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7)48T/96TY12371Homo sapiens (Human)
成纖維細胞生長因子受體2(FGFR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor Receptor 2 (FGFR2)48T/96TY12372Homo sapiens (Human)
羧肽酶A2(CPA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxypeptidase A2, Pancreatic (CPA2)48T/96TY12373Rattus norvegicus (Rat)
羧肽酶A1(CPA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxypeptidase A1, Pancreatic (CPA1)48T/96TY12374Rattus norvegicus (Rat)
間皮素(MSLN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Mesothelin (MSLN)48T/96TY12375Homo sapiens (Human)
V-Ets骨髓成紅細胞增多癥病毒E26癌基因同源物1(ETS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1)48T/96TY12376Mus musculus (Mouse)
顆粒體蛋白(GRN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Granulin (GRN)48T/96TY12377Homo sapiens (Human)
分泌型卷曲相關蛋白4(SFRP4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Secreted Frizzled Related Protein 4 (SFRP4)48T/96TY12378Mus musculus (Mouse)
視錐蛋白樣蛋白1(VSNL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Visinin Like Protein 1 (VSNL1)48T/96TY12379Homo sapiens (Human)
絲甘蛋白聚糖(SRGN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Serglycin (SRGN)48T/96TY12380Homo sapiens (Human)
Ⅷ型膠原α1(COL8α1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type VIII Alpha 1 (COL8a1)48T/96TY12381Homo sapiens (Human)
甲狀旁腺激素(PTH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY12382Canis familiaris; Canine (Dog)
低氧上調節因子1(HYOU1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hypoxia Up Regulated 1 (HYOU1)48T/96TY12383Homo sapiens (Human)
視黃醇結合蛋白4(RBP4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4)48T/96TY12384Bos taurus; Bovine (Cattle)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。