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小鼠抗小核糖核蛋白/Sm抗體酶免試劑盒,(snRNP/Sm)ELISA檢測試劑盒 ,英文名:snRNP/Sm ELISA Kit ,英文縮寫:snRNP/Sm ,常見試劑盒規格:96T/48T,產品別名:小鼠抗小核糖核蛋白/Sm抗體ELISA檢測試劑盒,snRNP/Sm 檢測試劑盒, snRNP/Sm 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠抗小核糖核蛋白/Sm抗體酶免試劑盒,(snRNP/Sm)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
生長分化因子5(GDF5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 5 (GDF5)48T/96TY12435Homo sapiens (Human)
羧肽酶E(CPE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxypeptidase E (CPE)48T/96TY12436Homo sapiens (Human)
白介素23受體(IL23R)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 23 Receptor (IL23R)48T/96TY12437Homo sapiens (Human)
TATA框結合蛋白關聯因子12(TAF12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for TATA Box Binding Protein Associated Factor 12 (TAF12)48T/96TY12438Mus musculus (Mouse)
精氨酸/絲氨酸豐富剪接因子4(SRSF4))檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Serine/Arginine Rich Splicing Factor 4 (SRSF4)48T/96TY12439Mus musculus (Mouse)
蛋白磷酸酶3調節因子亞基1(PPP3R1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1)48T/96TY12440Mus musculus (Mouse)
N-甲基-D-天氡氨酸離子能谷氨酸受體2A(GRIN2A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A)48T/96TY12441Mus musculus (Mouse)
組氨酸豐富糖蛋白(HRG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Histidine Rich Glycoprotein (HRG)48T/96TY12442Mus musculus (Mouse)
p53上調凋亡調節因子(PUMA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for p53 Upregulated Modulator Of Apoptosis (PUMA)48T/96TY12443Rattus norvegicus (Rat)
T-細胞可誘導共刺激分子(ICOS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Inducible T-Cell Co Stimulator (ICOS)48T/96TY12444Homo sapiens (Human)
分泌型卷曲相關蛋白5(SFRP5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)48T/96TY12445Homo sapiens (Human)
重肽鐵蛋白(FTH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ferritin, Heavy Polypeptide (FTH)48T/96TY12446Homo sapiens (Human)
2',5'-寡腺苷酸合成酶1(OAS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 2',5'-Oligoadenylate Synthetase 1 (OAS1)48T/96TY12447Homo sapiens (Human)
白介素22受體α2(IL2Rα2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 22 Receptor Alpha 2 (IL22Ra2)48T/96TY12448Rattus norvegicus (Rat)
腫廇蛋白P63(TP63)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Protein P63 (TP63)48T/96TY12449Homo sapiens (Human)
橋粒芯膠粘蛋白1(DSC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Desmocollin 1 (DSC1)48T/96TY12450Homo sapiens (Human)
夏科雷登氏結晶蛋白(CLC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Charcot Leyden Crystal Protein (CLC)48T/96TY12451Homo sapiens (Human)
防御素α3(DEFα3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Defensin Alpha 3, Neutrophil Specific (DEFa3)48T/96TY12452Homo sapiens (Human)
卵泡抑素樣蛋白1(FSTL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Follistatin Like Protein 1 (FSTL1)48T/96TY12453Homo sapiens (Human)
血小板反應蛋白2(THBS2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thrombospondin 2 (THBS2)48T/96TY12454Rattus norvegicus (Rat)
晶狀體蛋白αB(CRYαB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Crystallin Alpha B (CRYaB)48T/96TY12455Mus musculus (Mouse)
晶狀體蛋白αB(CRYαB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Crystallin Alpha B (CRYaB)48T/96TY12456Homo sapiens (Human)
解整合素金屬蛋白酶10(ADAM10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 10 (ADAM10)48T/96TY12457Rattus norvegicus (Rat)
血小板衍生生長因子受體樣蛋白(PDGFRL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor Receptor Like Protein (PDGFRL)48T/96TY12458Homo sapiens (Human)
血小板衍生生長因子受體樣蛋白(PDGFRL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor Receptor Like Protein (PDGFRL)48T/96TY12459Mus musculus (Mouse)
饑餓素(GHRL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ghrelin (GHRL)48T/96TY12460Sus scrofa; Porcine (Pig)
血管生成素樣蛋白4(ANGPTL4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 4 (ANGPTL4)48T/96TY12461Mus musculus (Mouse)
著絲粒蛋白F(CENPF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Centromere Protein F (CENPF)48T/96TY12462Homo sapiens (Human)
神經降壓素(NT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neurotensin (NT)48T/96TY12463Rattus norvegicus (Rat)
芳香酶(ARO)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aromatase (ARO)48T/96TY12464Rattus norvegicus (Rat)
分泌型卷曲相關蛋白5(SFRP5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)48T/96TY12465Mus musculus (Mouse)
腺瘤息肉病大腸桿菌蛋白(APC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Adenomatosis Polyposis Coli Protein (APC)48T/96TY12466Homo sapiens (Human)
Sestrin 2蛋白(SESN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sestrin 2 (SESN2)48T/96TY12467Mus musculus (Mouse)
前腦啡肽(PENK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Proenkephalin (PENK)48T/96TY12468Mus musculus (Mouse)
胰島素樣蛋白5(INSL5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Protein 5 (INSL5)48T/96TY12469Homo sapiens (Human)
脯氨酸豐富蛋白4(PRR4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Proline Rich Protein 4, Lacrimal (PRR4)48T/96TY12470Homo sapiens (Human)
Sestrin 3蛋白(SESN3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sestrin 3 (SESN3)48T/96TY12471Mus musculus (Mouse)
Sestrin 1蛋白(SESN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sestrin 1 (SESN1)48T/96TY12472Mus musculus (Mouse)
Jun B原癌基因(JUNB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Jun B Proto Oncogene (JUNB)48T/96TY12473Mus musculus (Mouse)
半胱氨酸蛋白酶抑制劑1(CST1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cystatin 1 (CST1)48T/96TY12474Homo sapiens (Human)
半胱氨酸蛋白酶抑制劑4(CST4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cystatin 4 (CST4)48T/96TY12475Homo sapiens (Human)
博來霉素水解酶(BLMH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Bleomycin Hydrolase (BLMH)48T/96TY12476Homo sapiens (Human)
去唾液酸糖蛋白受體2(ASGR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Asialoglycoprotein Receptor 2 (ASGR2)48T/96TY12477Homo sapiens (Human)
鎳紋蛋白(METRN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Meteorin (METRN)48T/96TY12478Homo sapiens (Human)
鎳紋蛋白(METRN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Meteorin (METRN)48T/96TY12479Mus musculus (Mouse)
Jagged 1蛋白(JAG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Jagged 1 Protein (JAG1)48T/96TY12480Homo sapiens (Human)
溶菌酶(LZM)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Lysozyme (LZM)48T/96TY12481Bos taurus; Bovine (Cattle)
Klotho蛋白(KL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Klotho (KL)48T/96TY12482Homo sapiens (Human)
短蛋白聚糖(BCAN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Brevican (BCAN)48T/96TY12483Homo sapiens (Human)
肝素酶(HPA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heparanase (HPA)48T/96TY12484Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。