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小鼠Qa淋巴細胞抗原2酶免試劑盒,(Qa-2)ELISA檢測試劑盒 ,英文名:Qa-2 ELISA Kit ,英文縮寫:Qa-2 ,常見試劑盒規格:96T/48T,產品別名:小鼠Qa淋巴細胞抗原2ELISA檢測試劑盒,Qa-2 檢測試劑盒, Qa-2 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠Qa淋巴細胞抗原2酶免試劑盒,(Qa-2)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
多聚蛋白2(MMRN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Multimerin 2 (MMRN2)48T/96TY13235Mus musculus (Mouse)
磷脂酰乙醇胺結合蛋白1(PEBP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phosphatidylethanolamine Binding Protein 1 (PEBP1)48T/96TY13236Homo sapiens (Human)
氫離子電壓門控通道蛋白1(HVCN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hydrogen Voltage Gated Channel Protein 1 (HVCN1)48T/96TY13237Homo sapiens (Human)
脯氨酸/絲氨酸豐富卷曲螺旋蛋白1(PSRC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Proline/Serine Rich Coiled Coil protein 1 (PSRC1)48T/96TY13238Homo sapiens (Human)
ATP結合盒轉運蛋白A3(ABCA3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for ATP Binding Cassette Transporter A3 (ABCA3)48T/96TY13239Homo sapiens (Human)
類胰蛋白酶β2(TPSβ2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tryptase Beta 2 (TPSb2)48T/96TY13240Homo sapiens (Human)
尿黑酸1,2-雙加氧酶(HGD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Homogentisate-1,2-Dioxygenase (HGD)48T/96TY13241Homo sapiens (Human)
羧肽酶N1(CPN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxypeptidase N1 (CPN1)48T/96TY13242Homo sapiens (Human)
蛋白酶體亞基β6(PSMβ6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Proteasome Subunit Beta Type 6 (PSMb6)48T/96TY13243Homo sapiens (Human)
轉位因子蛋白(TSPO)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Translocator Protein (TSPO)48T/96TY13244Homo sapiens (Human)
金屬硫蛋白3(MT3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Metallothionein 3 (MT3)48T/96TY13245Homo sapiens (Human)
安定結合抑制因子(DBI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Diazepam Binding Inhibitor (DBI)48T/96TY13246Homo sapiens (Human)
嗜鉻蛋白B(CHGB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chromogranin B (CHGB)48T/96TY13247Mus musculus (Mouse)
肝再生擴展生長因子(GFER)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Factor, Augmenter Of Liver Regeneration (GFER)48T/96TY13248Mus musculus (Mouse)
BMP激活素膜結合抑制因子同源物(BAMBI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI)48T/96TY13249Homo sapiens (Human)
跨膜蛋白2樣蛋白(TMEM2L)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transmembrane Protein 2 Like Protein (TMEM2L)48T/96TY13250Homo sapiens (Human)
半胱氨酸甘氨酸豐富蛋白1(CSRP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cysteine And Glycine Rich Protein 1 (CSRP1)48T/96TY13251Homo sapiens (Human)
二甲基精氨酸二甲胺水解酶1(DDAH1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dimethylarginine Dimethylaminohydrolase 1 (DDAH1)48T/96TY13252Homo sapiens (Human)
輔酶Q10同源物B(COQ10B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Coenzyme Q10 Homolog B (COQ10B)48T/96TY13253Homo sapiens (Human)
Jagged 1蛋白(JAG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Jagged 1 Protein (JAG1)48T/96TY13254Mus musculus (Mouse)
V-Rel網狀內皮增生病毒癌基因同源物B(RELB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB)48T/96TY13255Homo sapiens (Human)
成纖維細胞生長因子13(FGF13)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 13 (FGF13)48T/96TY13256Mus musculus (Mouse)
10kDa熱休克蛋白1(HSP10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heat Shock 10kDa Protein 1 (HSP10)48T/96TY13257Mus musculus (Mouse)
組蛋白H4(H4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Histone H4 (H4)48T/96TY13258Mus musculus (Mouse)
利鈉肽受體1(NPR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Natriuretic Peptide Receptor 1 (NPR1)48T/96TY13259Homo sapiens (Human)
利鈉肽受體3(NPR3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Natriuretic Peptide Receptor 3 (NPR3)48T/96TY13260Homo sapiens (Human)
角蛋白2(KRT2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Keratin 2 (KRT2)48T/96TY13261Homo sapiens (Human)
膽鹽依賴性脂肪酶(BSDL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lipase, Bile Salt Dependent (BSDL)48T/96TY13262Homo sapiens (Human)
腎上腺素能受體α2C(ADRα2C)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Adrenergic Receptor Alpha 2C (ADRa2C)48T/96TY13263Rattus norvegicus (Rat)
羧基端結合蛋白2(CTBP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for C-Terminal Binding Protein 2 (CTBP2)48T/96TY13264Homo sapiens (Human)
胞裂蛋白12(SEPT12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Septin 12 (SEPT12)48T/96TY13265Homo sapiens (Human)
十四烷基化丙氨酸豐富蛋白激酶C底物(MARCKS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS)48T/96TY13266Homo sapiens (Human)
巨軸索神經蛋白(GAN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gigaxonin (GAN)48T/96TY13267Homo sapiens (Human)
周期素依賴性激酶抑制因子3(CDKN3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase Inhibitor 3 (CDKN3)48T/96TY13268Homo sapiens (Human)
多聚免疫球蛋白受體(PIGR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Polymeric Immunoglobulin Receptor (PIGR)48T/96TY13269Mus musculus (Mouse)
11-β-羥基類固醇脫氫酶1(HSD11β1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)48T/96TY13270Rattus norvegicus (Rat)
神經肽Y受體Y5(NPY5R)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neuropeptide Y Receptor Y5 (NPY5R)48T/96TY13271Mus musculus (Mouse)
胃動蛋白2(GKN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gastrokine 2 (GKN2)48T/96TY13272Homo sapiens (Human)
脂多糖結合蛋白(LBP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lipopolysaccharide Binding Protein (LBP)48T/96TY13273Bos taurus; Bovine (Cattle)
Gremlin 1蛋白(GREM1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gremlin 1 (GREM1)48T/96TY13274Mus musculus (Mouse)
丁酰膽堿酯酶(BCHE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Butyrylcholinesterase (BCHE)48T/96TY13275Homo sapiens (Human)
突觸融合蛋白2(STX2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Syntaxin 2 (STX2)48T/96TY13276Mus musculus (Mouse)
突觸融合蛋白1A(STX1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Syntaxin 1A, Brain (STX1A)48T/96TY13277Mus musculus (Mouse)
谷氨酸半胱氨酸連接酶催化亞基(GCLC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutamate Cysteine Ligase, Catalytic (GCLC)48T/96TY13278Mus musculus (Mouse)
Bcl2樣蛋白11(BCL2L11)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Bcl2 Like Protein 11 (BCL2L11)48T/96TY13279Homo sapiens (Human)
基質金屬蛋白酶25(MMP25)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 25 (MMP25)48T/96TY13280Homo sapiens (Human)
Ⅵ型膠原α3(COL6α3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Collagen Type VI Alpha 3 (COL6a3)48T/96TY13281Homo sapiens (Human)
轉化受體電位陽離子通道亞家族M成員1(TRPM1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily M, Member 1 (TRPM1)48T/96TY13282Homo sapiens (Human)
成纖維細胞生長因子18(FGF18)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 18 (FGF18)48T/96TY13283Homo sapiens (Human)
彈性蛋白酶3B(ELA3B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Elastase 3B (ELA3B)48T/96TY13284Homo sapiens (Human)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。