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小鼠骨成型蛋白2酶免試劑盒,(BMP-2)ELISA檢測試劑盒,英文名:BMP-2 ELISA Kit,英文縮寫:BMP-2 ,常見試劑盒規格:96T/48T,產品別名:小鼠骨成型蛋白2ELISA檢測試劑盒,BMP-2 檢測試劑盒,BMP-2 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠骨成型蛋白2酶免試劑盒,(BMP-2)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
干擾素誘導T-細胞α亞族趨化劑(ITαC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Inducible T-Cell Alpha Chemoattractant (ITaC)48T/96TY13785Bos taurus; Bovine (Cattle)
SATB同源框蛋白1(SATB1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for SATB Homeobox Protein 1 (SATB1)48T/96TY13786Homo sapiens (Human)
乳酸脫氫酶C(LDHC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lactate Dehydrogenase C (LDHC)48T/96TY13787Homo sapiens (Human)
CUB帶狀瘡疹透明區樣域蛋白1(CUZD1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for CUB And Zona Pellucida Like Domains Protein 1 (CUZD1)48T/96TY13788Homo sapiens (Human)
貓眼綜合征染色體區候選基因1(CECR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1)48T/96TY13789Homo sapiens (Human)
生長停滯特異性蛋白6(GAS6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Arrest Specific Protein 6 (GAS6)48T/96TY13790Homo sapiens (Human)
信號素3E(SEMA3E)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Semaphorin 3E (SEMA3E)48T/96TY13791Homo sapiens (Human)
抗凝血酶(AT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Antithrombin (AT)48T/96TY13792Homo sapiens (Human)
絨毛蛋白1(VIL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Villin 1 (VIL1)48T/96TY13793Homo sapiens (Human)
前列腺干細胞抗原(PSCA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prostate Stem Cell Antigen (PSCA)48T/96TY13794Homo sapiens (Human)
前胸腺素α(PTMα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prothymosin Alpha (PTMa)48T/96TY13795Homo sapiens (Human)
Kruppel樣因子1(KLF1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kruppel Like Factor 1, Erythroid (KLF1)48T/96TY13796Mus musculus (Mouse)
鈉/氫交換因子1(NHE1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sodium/Hydrogen Exchanger 1 (NHE1)48T/96TY13797Homo sapiens (Human)
不均一核核糖核蛋白K(HNRPK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heterogeneous Nuclear Ribonucleoprotein K (HNRPK)48T/96TY13798Homo sapiens (Human)
甘油磷酸肌醇錨定高密度脂蛋白結合蛋白1(GPIHBP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1 (GPIHBP1)48T/96TY13799Homo sapiens (Human)
胰島素樣蛋白3(INSL3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Protein 3 (INSL3)48T/96TY13800Mus musculus (Mouse)
NEL樣蛋白2(NELL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for NEL Like Protein 2 (NELL2)48T/96TY13801Homo sapiens (Human)
角蛋白14(KRT14)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Keratin 14 (KRT14)48T/96TY13802Homo sapiens (Human)
谷氨酰胺果糖-6-磷酸轉氨酶2(GFPT2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutamine Fructose-6-Phosphate Transaminase 2 (GFPT2)48T/96TY13803Homo sapiens (Human)
二肽基肽酶6(DPP6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dipeptidyl Peptidase 6 (DPP6)48T/96TY13804Homo sapiens (Human)
反式高爾基體網狀結構蛋白2(TGOLN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Trans Golgi Network Protein 2 (TGOLN2)48T/96TY13805Homo sapiens (Human)
內凝集蛋白2(ITLN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Inectin 2 (ITLN2)48T/96TY13806Homo sapiens (Human)
鋅指同源框蛋白1B(ZFHX1B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Zinc Finger Homeobox Protein 1B (ZFHX1B)48T/96TY13807Mus musculus (Mouse)
DnaJ/Hsp40同源物亞家族C成員12(DNAJC12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12)48T/96TY13808Homo sapiens (Human)
殺傷細胞免疫球蛋白樣受體3DL1(KIR3DL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1)48T/96TY13809Homo sapiens (Human)
節律晝夜蛋白1(PER1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Period Circadian Protein 1 (PER1)48T/96TY13810Homo sapiens (Human)
Clock同源物(CLOCK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Clock Homolog (CLOCK)48T/96TY13811Homo sapiens (Human)
轉膠蛋白(TAGLN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transgelin (TAGLN)48T/96TY13812Homo sapiens (Human)
隱花色素1(CRY1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cryptochrome 1 (CRY1)48T/96TY13813Homo sapiens (Human)
配對框基因9(PAX9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Paired Box Gene 9 (PAX9)48T/96TY13814Homo sapiens (Human)
過氧化還原酶4(PRDX4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Peroxiredoxin 4 (PRDX4)48T/96TY13815Mus musculus (Mouse)
X-射線修復交叉互補蛋白6(XRCC6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for X-Ray Repair Cross Complementing 6 (XRCC6)48T/96TY13816Mus musculus (Mouse)
DNA修復蛋白RAD50(RAD50)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for DNA Repair Protein RAD50 (RAD50)48T/96TY13817Mus musculus (Mouse)
髓細胞觸發受體1(IREM1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Immune Receptor Expressed On Myeloid Cells 1 (IREM1)48T/96TY13818Homo sapiens (Human)
印度刺猬因子(IHH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hedgehog Homolog, Indian (IHH)48T/96TY13819Mus musculus (Mouse)
POTE錨蛋白域家族成員G(POTEG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for POTE Ankyrin Domain Family, Member G (POTEG)48T/96TY13820Homo sapiens (Human)
Neudesin神經營養因子(NENF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neudesin Neurotrophic Factor (NENF)48T/96TY13821Homo sapiens (Human)
細胞色素P450家族成員7A1(CYP7A1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cytochrome P450 7A1 (CYP7A1)48T/96TY13822Mus musculus (Mouse)
高遷移率族AT Hook蛋白1(HMGA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for High Mobility Group AT Hook Protein 1 (HMGA1)48T/96TY13823Canis familiaris; Canine (Dog)
白介素3受體α(IL3Rα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 3 Receptor Alpha (IL3Ra)48T/96TY13824Homo sapiens (Human)
神經肽Y受體Y1(NPY1R)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neuropeptide Y Receptor Y1 (NPY1R)48T/96TY13825Rattus norvegicus (Rat)
神經肽Y受體Y2(NPY2R)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neuropeptide Y Receptor Y2 (NPY2R)48T/96TY13826Rattus norvegicus (Rat)
白介素17D(IL17D)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 17D (IL17D)48T/96TY13827Homo sapiens (Human)
肽基精氨酸脫亞氨酶Ⅳ(PADI4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Peptidyl Arginine Deiminase Type IV (PADI4)48T/96TY13828Homo sapiens (Human)
基質金屬蛋白酶16(MMP16)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 16 (MMP16)48T/96TY13829Homo sapiens (Human)
封閉蛋白4(CLDN4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Claudin 4 (CLDN4)48T/96TY13830Rattus norvegicus (Rat)
高爾基蛋白73(GP73)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Golgi Protein 73 (GP73)48T/96TY13831Mus musculus (Mouse)
信號素4D(SEMA4D)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Semaphorin 4D (SEMA4D)48T/96TY13832Homo sapiens (Human)
信號淋巴細胞激活分子家族成員7(SLAMF7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7)48T/96TY13833Homo sapiens (Human)
叢狀蛋白B1(PLXNB1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plexin B1 (PLXNB1)48T/96TY13834Homo sapiens (Human)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。