服務熱線
021-54479081
021-54461587
產品展示PRODUCTS
本產品僅用于科研,不用于臨床
小鼠骨成型蛋白4酶免試劑盒,(BMP-4)ELISA檢測試劑盒,英文名:BMP-4 ELISA Kit,英文縮寫:BMP-4 ,常見試劑盒規格:96T/48T,產品別名:小鼠骨成型蛋白4ELISA檢測試劑盒,BMP-4 檢測試劑盒,BMP-4 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠骨成型蛋白4酶免試劑盒,(BMP-4)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
食道癌相關基因4(ECRG4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Esophageal Cancer Related Gene 4 (ECRG4)48T/96TY13835Homo sapiens (Human)
泛素交叉反應蛋白(UCRP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ubiquitin Cross Reactive Protein (UCRP)48T/96TY13836Homo sapiens (Human)
細胞球蛋白(CYGB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cytoglobin (CYGB)48T/96TY13837Rattus norvegicus (Rat)
內皮細胞粘附分子(ESAM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Endothelial Cell Adhesion Molecule (ESAM)48T/96TY13838Homo sapiens (Human)
Epigen蛋白(EPG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Epigen (EPG)48T/96TY13839Homo sapiens (Human)
雙氫睪酮(DHT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dihydrotestosterone (DHT)48T/96TY13840General
甘露糖受體C2(MRC2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Mannose Receptor C Type 2 (MRC2)48T/96TY13841Mus musculus (Mouse)
防御素β103B(DEFβ103B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Defensin Beta 103B (DEFB103B)48T/96TY13842Homo sapiens (Human)
血小板激活因子(PAF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plaet Activating Factor (PAF)48T/96TY13843Bos taurus; Bovine (Cattle)
肝型脂肪酸結合蛋白(FABP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 1, Liver (FABP1)48T/96TY13844Homo sapiens (Human)
黃嘌呤脫氫酶(XDH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Xanthine Dehydrogenase (XDH)48T/96TY13845Bos taurus; Bovine (Cattle)
白介素1η(IL1η)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 1 Eta (IL1h)48T/96TY13846Homo sapiens (Human)
卷曲同源物6(FZD6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Frizzled Homolog 6 (FZD6)48T/96TY13847Homo sapiens (Human)
富酪蛋白(STATH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Statherin (STATH)48T/96TY13848Homo sapiens (Human)
整合素α1(ITGα1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Integrin Alpha 1 (ITGa1)48T/96TY13849Mus musculus (Mouse)
細胞因子信號轉導抑制因子1(SOCS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Suppressors Of Cytokine Signaling 1 (SOCS1)48T/96TY13850Homo sapiens (Human)
CD200受體1(CD200R1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for CD200 Receptor 1 (CD200R1)48T/96TY13851Homo sapiens (Human)
二酰甘油-O-酰基轉移酶同源物1(DGAT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1)48T/96TY13852Mus musculus (Mouse)
Wolfram綜合征蛋白1(WFS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Wolfram Syndrome Protein 1 (WFS1)48T/96TY13853Homo sapiens (Human)
Wolfram綜合征蛋白1(WFS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Wolfram Syndrome Protein 1 (WFS1)48T/96TY13854Mus musculus (Mouse)
Wolfram綜合征蛋白1(WFS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Wolfram Syndrome Protein 1 (WFS1)48T/96TY13855Rattus norvegicus (Rat)
表面活性物質關聯蛋白J(SPJ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Surfactant Associated Protein J (SPJ)48T/96TY13856Rattus norvegicus (Rat)
白介素25(IL25)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 25 (IL25)48T/96TY13857Rattus norvegicus (Rat)
可溶性半乳糖凝集素3結合蛋白(LGALS3BP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP)48T/96TY13858Mus musculus (Mouse)
ⅩⅤ型膠原(COL15)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type XV (COL15)48T/96TY13859Mus musculus (Mouse)
細胞外基質蛋白1(ECM1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Extracellular Matrix Protein 1 (ECM1)48T/96TY13860Mus musculus (Mouse)
封閉蛋白3(CLDN3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Claudin 3 (CLDN3)48T/96TY13861Rattus norvegicus (Rat)
封閉蛋白5(CLDN5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Claudin 5 (CLDN5)48T/96TY13862Rattus norvegicus (Rat)
多胺調節因子1結合蛋白1(PMFBP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1)48T/96TY13863Homo sapiens (Human)
載脂蛋白O(APOO)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apolipoprotein O (APOO)48T/96TY13864Homo sapiens (Human)
纖維鞘相互作用蛋白1(FSIP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibrous Sheath Interacting Protein 1 (FSIP1)48T/96TY13865Homo sapiens (Human)
連接附著分子3(JAM3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Junctional Adhesion Molecule 3 (JAM3)48T/96TY13866Homo sapiens (Human)
連接附著分子1(JAM1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Junctional Adhesion Molecule 1 (JAM1)48T/96TY13867Homo sapiens (Human)
中間絲蛋白(SYNC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Syncoilin (SYNC)48T/96TY13868Homo sapiens (Human)
抵抗素樣蛋白β(RETNLβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Resistin Like Beta (RETNLb)48T/96TY13869Homo sapiens (Human)
毒蕈堿型膽堿受體M2(CHRM2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Muscarinic 2 (CHRM2)48T/96TY13870Mus musculus (Mouse)
毒蕈堿型膽堿受體M2(CHRM2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Muscarinic 2 (CHRM2)48T/96TY13871Rattus norvegicus (Rat)
胸腺基質淋巴細胞生成素(TSLP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thymic Stromal Lymphopoietin (TSLP)48T/96TY13872Mus musculus (Mouse)
L1-細胞粘附分子(L1CAM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for L1-Cell Adhesion Molecule (L1CAM)48T/96TY13873Mus musculus (Mouse)
天冬酰胺合成酶(ASNS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Asparagine Synthetase (ASNS)48T/96TY13874Homo sapiens (Human)
白細胞彈性蛋白酶抑制因子(LEI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Leukocyte Elastase Inhibitor (LEI)48T/96TY13875Mus musculus (Mouse)
蛋白酪氨酸磷酸酶受體G(PTPRG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase Receptor Type G (PTPRG)48T/96TY13876Homo sapiens (Human)
興奮性氨基酸轉運蛋白1(EAAT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Excitatory Amino Acid Transporter 1 (EAAT1)48T/96TY13877Homo sapiens (Human)
驅動蛋白家族成員5A(KIF5A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kinesin Family, Member 5A (KIF5A)48T/96TY13878Homo sapiens (Human)
驅動蛋白家族成員18A(KIF18A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kinesin Family, Member 18A (KIF18A)48T/96TY13879Homo sapiens (Human)
G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for G Protein Coupled Estrogen Receptor 1 (GPER)48T/96TY13880Homo sapiens (Human)
Bcl2相關髓細胞白血病序列1(MCL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1)48T/96TY13881Homo sapiens (Human)
IgG-Fc片段結合蛋白(FcγBP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fc Fragment Of IgG Binding Protein (FcgBP)48T/96TY13882Homo sapiens (Human)
序列相似家族132成員A(FAM132A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Family With Sequence Similarity 132, Member A (FAM132A)48T/96T Y13883Homo sapiens (Human)
形成素結合蛋白1(FNBP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Formin Binding Protein 1 (FNBP1)48T/96TY13884Homo sapiens (Human)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。