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小鼠血管生成素4酶免試劑盒,(ANG-4)ELISA檢測試劑盒 ,英文名:ANG-4 ELISA Kit ,英文縮寫:ANG-4 ,常見試劑盒規格:96T/48T,產品別名:小鼠血管生成素4ELISA檢測試劑盒,ANG-4 檢測試劑盒, ANG-4 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠血管生成素4酶免試劑盒,(ANG-4)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
脂肪酶K(LIPK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lipase K (LIPK)48T/96TY14435Homo sapiens (Human)
脂肪酶N(LIPN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lipase N (LIPN)48T/96TY14436Homo sapiens (Human)
脂肪酶M(LIPM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lipase M (LIPM)48T/96TY14437Homo sapiens (Human)
CopineⅧ蛋白(CPNE8)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Copine VIII (CPNE8)48T/96TY14438Homo sapiens (Human)
Paladin蛋白(PALD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Paladin (PALD)48T/96TY14439Homo sapiens (Human)
環氧化合物水解酶4(EPHX4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Epoxide Hydrolase 4 (EPHX4)48T/96TY14440Homo sapiens (Human)
環氧化合物水解酶3(EPHX3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Epoxide Hydrolase 3 (EPHX3)48T/96TY14441Homo sapiens (Human)
磷脂酶D4(PLD4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phospholipase D4 (PLD4)48T/96TY14442Homo sapiens (Human)
Serrate蛋白(SRRT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Serrate (SRRT)48T/96TY14443Homo sapiens (Human)
突觸囊泡蛋白ⅩⅦ(SYT17)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Synaptotagmin XVII (SYT17)48T/96TY14444Homo sapiens (Human)
酵母氨酸脫氫酶(SCCPDH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Saccharopine Dehydrogenase (SCCPDH)48T/96TY14445Homo sapiens (Human)
CopineⅨ蛋白(CPNE9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Copine IX (CPNE9)48T/96TY14446Homo sapiens (Human)
N-α-乙酰轉移酶35(NαA35)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Alpha-Acetyltransferase 35 (NaA35)48T/96TY14447Homo sapiens (Human)
Tectonic 3蛋白(TCTN3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tectonic 3 (TCTN3)48T/96TY14448Homo sapiens (Human)
延伸蛋白A3(ELOA3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Elongin A3 (ELOA3)48T/96TY14449Homo sapiens (Human)
Metaxin 3蛋白(MTX3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Metaxin 3 (MTX3)48T/96TY14450Homo sapiens (Human)
軸突生長誘向因子5(Ntn5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Netrin 5 (Ntn5)48T/96TY14451Homo sapiens (Human)
假尿苷酸合酶3(PUS3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pseudouridylate Synthase 3 (PUS3)48T/96TY14452Homo sapiens (Human)
Anoctamin 10蛋白(ANO10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Anoctamin 10 (ANO10)48T/96TY14453Homo sapiens (Human)
Akirin 1蛋白(AKIRIN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Akirin 1 (AKIRIN1)48T/96TY14454Homo sapiens (Human)
Tectonic 2蛋白(TCTN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tectonic 2 (TCTN2)48T/96TY14455Homo sapiens (Human)
N-α-乙酰轉移酶40(NαA40)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Alpha-Acetyltransferase 40 (NaA40)48T/96TY14456Homo sapiens (Human)
核仁蛋白10(0)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleolar Protein 10 (0)48T/96TY14457Homo sapiens (Human)
N-乙酰轉移酶15(NAT15)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Acetyltransferase 15 (NAT15)48T/96TY14458Homo sapiens (Human)
羰基還原酶4(CBR4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carbonyl Reductase 4 (CBR4)48T/96TY14459Homo sapiens (Human)
肌醇單磷酸酶3(IMPA3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Inositol Monophosphatase 3 (IMPA3)48T/96TY14460Homo sapiens (Human)
假尿苷酸合酶7(PUS7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pseudouridylate Synthase 7 (PUS7)48T/96TY14461Homo sapiens (Human)
肌球蛋白ⅩⅨ(MYO19)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myosin XIX (MYO19)48T/96TY14462Homo sapiens (Human)
核仁蛋白9(NOL9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleolar Protein 9 (NOL9)48T/96TY14463Homo sapiens (Human)
Raftlin 2蛋白(RFTN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Raftlin 2 (RFTN2)48T/96TY14464Homo sapiens (Human)
羧酸酯酶5A(CES5A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxylesterase 5A (CES5A)48T/96TY14465Homo sapiens (Human)
腺苷酸激酶8(AK8)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Adenylate Kinase 8 (AK8)48T/96TY14466Homo sapiens (Human)
筑絲蛋白5(TEKT5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tektin 5 (TEKT5)48T/96TY14467Homo sapiens (Human)
肌間蛋白3(MYOM3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myomesin 3 (MYOM3)48T/96TY14468Homo sapiens (Human)
羧酸酯酶4A(CES4A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxylesterase 4A (CES4A)48T/96TY14469Homo sapiens (Human)
磷脂酶D5(PLD5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phospholipase D5 (PLD5)48T/96TY14470Homo sapiens (Human)
羥基丙酮酸異構酶(HYI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hydroxypyruvate Isomerase (HYI)48T/96TY14471Homo sapiens (Human)
甘油磷酸膽堿磷酸二酯酶(GPCPD1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycerophosphocholine Phosphodiesterase (GPCPD1)48T/96TY14472Homo sapiens (Human)
羧肽酶X2(CPX2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxypeptidase X2 (CPX2)48T/96TY14473Homo sapiens (Human)
紡錘體蛋白4(SPIN4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Spindlin 4 (SPIN4)48T/96TY14474Homo sapiens (Human)
紡錘體蛋白3(SPIN3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Spindlin 3 (SPIN3)48T/96TY14475Homo sapiens (Human)
Fritz蛋白(FRTZ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fritz (FRTZ)48T/96TY14476Homo sapiens (Human)
古梅斯蛋白2(AMZ2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Archaemetzincin 2 (AMZ2)48T/96TY14477Homo sapiens (Human)
X-脯氨酰氨肽酶3(XPNPEP3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for X-Prolyl Aminopeptidase 3 (XPNPEP3)48T/96TY14478Homo sapiens (Human)
N-α-乙酰轉移酶11(NαA11)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Alpha-Acetyltransferase 11 (NaA11)48T/96TY14479Homo sapiens (Human)
皂素(Haponin)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Haponin48T/96TY14480Homo sapiens (Human)
孤兒素磷酸酶2(PHOSPHO2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phosphatase, Orphan 2 (PHOSPHO2)48T/96TY14481Homo sapiens (Human)
核仁蛋白12(2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleolar Protein 12 (2)48T/96TY14482Homo sapiens (Human)
甘油激酶5(GK5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycerol Kinase 5 (GK5)48T/96TY14483Homo sapiens (Human)
磷酸甘油酸變位酶5(PGAM5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phosphoglycerate Mutase 5 (PGAM5)48T/96TY14484Homo sapiens (Human)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。