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小鼠α葡萄糖苷酶酶免試劑盒,(a-Glu)ELISA檢測試劑盒 ,英文名:a-Glu ELISA Kit ,英文縮寫:a-Glu ,常見試劑盒規格:96T/48T,產品別名:小鼠α葡萄糖苷酶ELISA檢測試劑盒,a-Glu 檢測試劑盒, a-Glu 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠α葡萄糖苷酶酶免試劑盒,(a-Glu)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
胃泌素釋放肽(GRP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gastrin Releasing Peptide (GRP)48T/96TY16285Homo sapiens (Human)
羥烷基輔酶A脫氫酶β(HADHβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb)48T/96TY16286Rattus norvegicus (Rat)
碳酸酐酶Ⅻ(CA12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase XII (CA12)48T/96TY16287Homo sapiens (Human)
細胞色素P450家族成員11A1(CYP11A1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cytochrome P450 11A1 (CYP11A1)48T/96TY16288Rattus norvegicus (Rat)
周期素依賴性激酶6(CDK6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase 6 (CDK6)48T/96TY16289Homo sapiens (Human)
腫瘤易感基因101(TSG101)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Susceptibility Gene 101 (TSG101)48T/96TY16290Homo sapiens (Human)
A類清道夫受體3(SCARA3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Scavenger Receptor Class A Member 3 (SCARA3)48T/96TY16291Mus musculus (Mouse)
淀粉樣前體蛋白(APP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Amyloid Precursor Protein (APP)48T/96TY16292Mus musculus (Mouse)
視紫質(RHO)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Rhodopsin (RHO)48T/96TY16293Mus musculus (Mouse)
N-乙酰轉移酶8樣蛋白(NAT8L)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Acetyltransferase 8 Like Protein (NAT8L)48T/96TY16294Homo sapiens (Human)
腱蛋白樣蛋白2(CHRDL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chordin Like Protein 2 (CHRDL2)48T/96TY16295Mus musculus (Mouse)
白介素4(IL4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 4 (IL4)48T/96TY16296Cavia (Guinea pig )
NADH脫氫酶1(ND1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for NADH Dehydrogenase 1 (ND1)48T/96TY16297Mus musculus (Mouse)
細胞色素C氧化酶亞基Ⅲ(COX3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cytochrome C Oxidase Subunit III (COX3)48T/96TY16298Mus musculus (Mouse)
脾臟酪氨酸激酶(SYK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Spleen Tyrosine Kinase (SYK)48T/96TY16299Homo sapiens (Human)
黑色素聚集激素受體1(MCHR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Melanin Concentrating Hormone Receptor 1 (MCHR1)48T/96TY16300Homo sapiens (Human)
δ樣蛋白1同源物(δLK1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Delta Like 1 Homolog (dLK1)48T/96TY16301Homo sapiens (Human)
重肽鐵蛋白(FTH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ferritin, Heavy Polypeptide (FTH)48T/96TY16302Rattus norvegicus (Rat)
線粒體鐵蛋白(FTMT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ferritin, Mitochondrial (FTMT)48T/96TY16303Rattus norvegicus (Rat)
二價金屬轉運蛋白1(DMT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Divalent Metal Transporter 1 (DMT1)48T/96TY16304Rattus norvegicus (Rat)
鐵反應元件結合蛋白2(IREB2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Iron Responsive Element Binding Protein 2 (IREB2)48T/96TY16305Rattus norvegicus (Rat)
順烏頭酸酶1(ACO1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aconitase 1 (ACO1)48T/96TY16306Rattus norvegicus (Rat)
蛋白酶激活受體1(PAR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protease Activated Receptor 1 (PAR1)48T/96TY16307Homo sapiens (Human)
谷氨酸半胱氨酸連接酶催化亞基(GCLC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutamate Cysteine Ligase, Catalytic (GCLC)48T/96TY16308Rattus norvegicus (Rat)
Mg2+/Mn2+依賴性蛋白磷酸酶1A(PPM1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A)48T/96TY16309Homo sapiens (Human)
Mg2+/Mn2+依賴性蛋白磷酸酶1A(PPM1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A)48T/96TY16310Rattus norvegicus (Rat)
組蛋白脫乙酰基酶1(HDAC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Histone Deacetylase 1 (HDAC1)48T/96TY16311Rattus norvegicus (Rat)
甲基多巴1β(MEP1β)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Meprin A Beta (MEP1b)48T/96TY16312Homo sapiens (Human)
Bcl2關聯X蛋白(Bax)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Bcl2 Associated X Protein (Bax)48T/96TY16313Mus musculus (Mouse)
血管生成素樣蛋白2(ANGPTL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 2 (ANGPTL2)48T/96TY16314Rattus norvegicus (Rat)
抗肌萎縮蛋白(UTRN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Utrophin (UTRN)48T/96TY16315Mus musculus (Mouse)
鈉/鉀離子轉運ATP酶β4肽(ATP1β4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4)48T/96TY16316Homo sapiens (Human)
UDP葡萄糖神經酰胺葡萄糖基轉移酶(UGCG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for UDP Glucose Ceramide Glucosyltransferase (UGCG)48T/96TY16317Mus musculus (Mouse)
半胱氨酸豐富血管生成誘導因子61(CYR61)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61)48T/96TY16318Mus musculus (Mouse)
UDP葡糖醛酸基轉移酶1家族多肽A1(UGT1A1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1)48T/96TY16319Homo sapiens (Human)
視網膜血管瘤腫瘤抑制因子(vHL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Von Hippel Lindau Tumor Suppressor (vHL)48T/96TY16320Rattus norvegicus (Rat)
細胞色素P450家族成員2B6(CYP2B6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cytochrome P450 2B6 (CYP2B6)48T/96TY16321Mus musculus (Mouse)
錨定蛋白重復域蛋白1(ANKRD1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ankyrin Repeat Domain Protein 1 (ANKRD1)48T/96TY16322Rattus norvegicus (Rat)
角蛋白18(KRT18)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Keratin 18 (KRT18)48T/96TY16323Mus musculus (Mouse)
雌激素受體β(ERβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Estrogen Receptor Beta (ERb)48T/96TY16324Mus musculus (Mouse)
5-羥色胺受體1A(HTR1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 5-Hydroxytryptamine Receptor 1A (HTR1A)48T/96TY16325Homo sapiens (Human)
毒蕈堿型膽堿受體M3(CHRM3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Muscarinic 3 (CHRM3)48T/96TY16326Mus musculus (Mouse)
糖蛋白A33(GPA33)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycoprotein A33 (GPA33)48T/96TY16327Rattus norvegicus (Rat)
IL2誘導T細胞激酶(ITK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for IL2 Inducible T-Cell Kinase (ITK)48T/96TY16328Mus musculus (Mouse)
CD5抗原樣蛋白(CD5L)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for CD5 Antigen Like Protein (CD5L)48T/96TY16329Homo sapiens (Human)
神經元正五聚蛋白Ⅰ(NPTX1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neuronal Pentraxin I (NPTX1)48T/96TY16330Mus musculus (Mouse)
維生素D結合蛋白(DBP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vitamin D Binding Protein (DBP)48T/96TY16331Mus musculus (Mouse)
尾型同源框轉錄因子2(CDX2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Caudal Type Homeobox Transcription Factor 2 (CDX2)48T/96TY16332Homo sapiens (Human)
肌侵蛋白(MTPN)檢測試劑盒(酶聯免疫吸附試驗法)小鼠α葡萄糖苷酶酶免試劑盒,(a-Glu)ELISA檢測試劑盒ELISA Kit for Myotrophin (MTPN)48T/96TY16333Mus musculus (Mouse)
阿米洛利敏感鈉離子通道蛋白1α(SCNN1α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a)48T/96TY16334Rattus norvegicus (Rat)
各種類型ELISA測定時,小鼠α葡萄糖苷酶酶免試劑盒,(a-Glu)ELISA檢測試劑盒一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。